Actinomycetes media without protein sources?

More Abdulrahman Alghamdi's questions See All

HPLC actinomyceyes active compound ?

I have actinomycetes extraction and I need to detected by HPLC, so I need the protocol of hplc RP-8 chromatographic column

11 December 2019 5,172 3 View

What is the best liquid media used to growing soil actinomycetes for extract the secondary metabolites?

I'm using a starch-casein broth , but in some caseies the starch still in the bottom of the flask.. so, I'm looking for a liquid media that used to growing soil actinomycetes for extract secondary...

06 July 2019 4,733 6 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Miguel Fernández-Niño

Hope u could adapt this one:

https://www.ncbi.nlm.nih.gov/m/pubmed/631889/

Abdulrahman Alghamdi

Miguel Fernández-Niño Thank you

Leandra Moller

You can try this one:

1.18g succinic acid

0.29g L-glutamine

0.2g CaCl2.2H2O

0.2g KH2PO4

0.2g MgSO4.7H2O

0.1g NaCl

0.09g m-inositol

0.037g ferric EDTA

4.5mg MnSO4.H2O

1.5mg H3BO3

1.5mg ZnSO4.H2O

0.5mg nicotinic acid

0.5mg piridoxine-HCl

0.1mg thiamine-HCl

0.04mg CuSO4.5H2O

0.025 mg Na2MoO4.2H2O

pH of 6.4 at room temperature

Add ingredients to distilled water and bring volume up to 1 liter. Mix and aliquot to vessels of choice. Autoclave as usual