I'm using a starch-casein broth , but in some caseies the starch still in the bottom of the flask.. so, I'm looking for a liquid media that used to growing soil actinomycetes for extract secondary metabolites.
If the Strain belongs to the genus Streptomyces, you can use Modified Nutrient Glucose (MNG) broth medium, which gives excellent results in secondary metabolites extraction with the optimal growth conditions.
Medium composition per liter as follows:
Glucose 10 gram
Peptone 5 gram
Yeast extract 3 gram
Beef extract 3 gram
NaCl 3 gram
1000 ml distilled water
pH 7
All the best
Soluble starch 2.0g, potassium nitrate 0.1g, dipotassium hydrogen phosphate 0.05g, sodium chloride 0.05g, magnesium sulfate 0.05g, ferrous sulfate 0.001g, agar 2g, Water 100ml.
First, put starch in beaker, mix 5 ml water into paste, pour 95 ml water, mix well and add other drugs to dissolve it. Make a mark outside the beaker, add agar when heated to boil, stirring constantly, until agar is completely dissolved, make up for water loss. Adjust the pH value to 7.2-7.4, sterilize after packing.
Please visit this site: http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.319.3529&rep=rep1&type=pdf. You can also use the International Streptomyces Project (ISP) broth medium. You are conducting a research, so play around with media types and compositions and come up with something most suitable for your work.
You can use modified minimal salt medium for the selective isolation of members of the Streptomyces. Please see this article
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts