I understand that a method of inoculating legume seedlings (e.g. Aisyah et al, 2013: Variation in accumulation of isoflavonoids in Phaseoleae seedlings elicited by Rhizopus) is to prepare a 0.2mL/g spore suspension containing aqueous NaCl and R. microsporus grown to 10^7 cfu/mL, which is to be added to the seeds. Should the seeds in the incubator be left with the mixture until the end of the incubation period? Or should the spore suspension be drained out after a set period of time?
For inoculation of legume seedlings with Rhizopus oryzae there are two methods. If we want to study under in vitro conditions, the legume seedings germinated on 1.0 sterilized agar plates may be dipped into fungal spores containing 105spores per ml. If we want to study their impact under pot house conditions then to germinated seed add 1.0ml of spore suspension per pot.
My Dear the following link will help you
https://www.ncbi.nlm.nih.gov/pubmed/22414936
Dear Sirs Pathak and al Tawaha
Thank you very much for your help. I'll look into this.
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