How will I calculate concentration of a compound in a crude extract by HPLC ?
If you know what the compound is you can prepare and analyze a standard of that compound at a known concentration to get its response versus concentration data, which can then be used to quantify the response of the compound in the sample. If you don't have a standard you can use another compound to provide a generic response, but there will be more uncertainty as to the accuracy of that value.
If you know beforehand the target analyte then develop calibration curve using it as a standard and then find out the concentration of analyst in sample. If you suspect it to be there confirm by internal and or external spiking.
Could you please suggest me any equation or pdf file that describes the calculation equation ?@ Steven A Zdravkovic Shrikaant Kulkarni
@Md. ahmudul Hasan, Yes spiking will help you ensure the presence or absence of the target and if present to what extent it is present based on peak area which is a measure of concentration.
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