Md. Mahmudul Hasan

6 Questions 19 Answers 0 Followers

Questions related from Md. Mahmudul Hasan

TE Buffer or DNase/RNase free water?

I purchased few primers for my experiment. I usually use DNase/RNasa free water for reconstituting the lyophilized primers. But, the manufacturer recommended TE Buffer on the body of primer tube....

04 November 2021 8,461 3 View

Which software is best for opening a large FASTA file (~300Mb) ?

I am working with an animal, which has no gene sequence in any well known database. One group has sequenced the transcriptoms for that animal; and they have their own database, where they made...

21 October 2021 9,213 3 View

Can I design primer using partial sequences of a gene ?

I want to study some gene expression of an animal, which genome sequence is not completely available. There are very limited data about the animal. But, there are some partial gene sequence. Can I...

19 October 2021 7,262 3 View

Is there any option for characterization of Silver Nano-particles (AgNP) in Bangladesh ?

I want to characterized Silver Nano-particles (AgNP) after green synthesis. Is any of following options - Scanning Calorimetry (DSC), Ultra Violet Visible Spectral (UV-vis) analysis, Fourier...

30 October 2020 5,841 3 View

What should I must do for HPLC method validation ?

Which parameters (among linearity, LOD, LOQ, %RSD, % of recovery etc.) I should must check in HPLC method validation ?

06 October 2020 5,210 25 View

How will I calculate concentration of a compound in a crude extract by HPLC ?

06 October 2020 3,202 5 View