How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Is BRSV ("bovine respiratory syncytial virus") less susceptible to RNA degradation than SARS-CoV-2 when thawing a sample?

I've noticed that when thawing long-frozen samples (3-4 months at -20 C), most of the SARS-CoV-2 virus does not remain intact, but that the surrogate appears to. Has anyone else noticed this?

09 January 2021 4,168 7 View

Can you centrifuge and re-suspend a cDNA sample?

I am woking with very low concentrations of RNA in an attempt to run a qPCR analysis. Given this, I am going to attempt to synthesize cDNA without diluting my sample, and in two different,...

12 December 2020 3,247 3 View

Is the "Releve" method still standard protocol for plant community sampling and percent cover estimation?

I am not a botanist but need to estimate the average percent vegetation cover of my study area. I have researched this and found articles that use the "releve" method for determining plot size...

22 September 2020 5,676 6 View

How to accelerate Langevin dynamics simulations for bead-spring polymer chain models?

I'm running simulations for a bead-spring polymer networks using LAMMPS. I need my simulations to reach biologically relevant timescales of at least a few milliseconds. However each simulation...

14 September 2020 2,954 1 View

Convex Optimization for Deep Learning?

Is there such a thing as convex optimization for large deep learning networks? Is there a way to guarantee convergence? Has anyone looked into this?

06 August 2020 5,384 4 View

What happens in post-secondary education when instructors don't use traditional grades?

I am looking for empirical studies that examine the use of non-traditional grading in higher education. This means NOT using A-F grades during a course (even if, at the conclusion of the course,...

09 June 2020 1,519 6 View

What is the role of journals' reviewers? Should they be absolute judges?

- Ask questions where they are not sure/convinced about a statement/conclusion VS reject a statement/conclusion where they 'think' it is not correct? - Provide suggestions to improve a...

21 May 2020 2,624 7 View

Looking for immunologist or virologist to help with literature-based study for COVID treatment. Can you help?

On the side, I doing some research (literature-based) on a particular treatment that may help for COVID-19 symptoms but I need someone with a stronger medical knowledge base with expertise in...

29 April 2020 8,098 11 View

Female Soccer - Small sided games - Does training intensity reduce when elite players train with non elite ? or senior players with lower ages groups?

Hi , Im wondering does anyone know of any papers detailing the effects to training intensity of small sided games when the group is of mixed performance levels i.e( professional& semi...

18 December 2019 6,495 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View