In tonsil we normally use CD3 and CD19 to help stain the overall cross section. If I was looking at a cross section of the liver, what antibodies should I use to help me see the overall strucutre?
Thanks!
anti-hepatocyte stains livercells. But for simple structure staining a trichrome-stains like Gömöri or Masson show liver-architecture very well.
Thanks! Any specific antibodies?
In tonsil we normally use CD3 and CD19 to help stain the overall cross section. If I was looking at a cross section of the liver, what antibodies should I use to help me see the overall...
08 September 2018 1,484 3 View
I've worked with human tonsil and brain but never with liver. Thanks!
15 August 2018 7,658 3 View
07 August 2018 1,383 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
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07 August 2024 1,028 0 View
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07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View