I need strains of Agrobacterium rhizogenes or Agrobacterium tumefaciens, how can I get them?

I need the above strains for my students .

02 October 2020 5,867 5 View

Transfection_large plasmid_HEK cells?

Does anyone have any experience with transfecting 12Kb plasmid in HEK cell line using jet prime?

07 November 2019 1,450 3 View

Hi Everyone. i wanted to ask about extraction of proteins from cell lines for SDS PAGE and further western blotting?

I take the cells trypsinize the cells and wash them with cold PBS two times. Then i use RIPA lysis buffer according to the pellet and add protease inhibitors 1/20th volume of lysis buffer. Pipet...

16 March 2017 3,517 2 View

Plasmid Extraction Problem?

03 February 2016 3,352 3 View

How can I interpret the Nyquist plot from EIS data?

12 February 2015 2,962 13 View

What should be the volume of each components of Ce(NO3)3, NH4Cl, and KCl to electrochemically deposit CeO2NW?

Thanks in advance for your replies.

03 January 2015 7,879 2 View

Can anyone recommend resources for searching inhibitors to a protein?

For docking studies of my protein I need inhibitors. I have already used Brenda(database) for this. Is there any other resource for inhibitor search?

25 July 2012 6,566 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Can someone help me with this error that I am getting while estimating a VAR model in R?

I am using annual time series data to estimate a VAR model in R. However, when I run the command to find the optimal lag length, I get the following error: Fehler in VARselect(data, lag.max = 10,...

18 February 2021 9,647 3 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View