https://www.researchgate.net/post/Can_someone_advise_on_how_to_estimate_the_number_of_trypanosomes_in_a_blood_sample
Thick or thin blood film: counting one hundred WBCs (or more), report the number of parasites per 100 WBCs on the smear. In some cases (especially malaria) quantification of parasites yields clinically useful information. To quantify malaria parasites against RBCs, count the parasitized RBCs among 500-2,000 RBCs on the thin smear and express the results as % parasitemia.
% parasitemia = (parasitized RBCs/total RBCs) × 100
If the parasitemia is high (e.g., > 10%) examine 500 RBCs; if it is low (e.g.,
Also, kindly check:
Article Quantitative Determination of Plasmodium Parasitemia by Flow...
https://academic.oup.com/cid/article/49/7/1103/316703
Dear Dr Asief Zihadi . See the following useful RG link: Article Identification of ticks and detection of blood protozoa in f...
Kindly see also the following useful RG link: Article Automated estimation of parasitaemia of Plasmodium yoelii-in...
Also check please the following very good RG link: Article The buffy coat method: a tool for detection of blood parasit...
The following RG link is also very useful : Article Quantification of hematozoa in blood smears
Dear Asief Zihadi
Check in our studies, we can provide answer by your question.
Good luck!
https://www.researchgate.net/publication/352383659_First_record_of_Trypanosoma_sp_Kinetoplastea_Trypanosomatidae_parasiting_Rhinella_major_in_the_Brazilian_Amazon
https://www.researchgate.net/publication/344750634_Morphometric_Characterization_of_Trypanosoma_spp_and_blood_parameters_in_Pterygoplichthys_pardalis_Pisces_Loricariidae_from_the_Brazilian_Amazon
https://www.researchgate.net/publication/343490492_Morphometric_and_morphological_characterization_of_Trypanosoma_sp_Kinetoplastea_Trypanosomatidae_hemoparasite_of_Crested_Eagle_Morphnus_guianensis_in_the_Brazilian_Amazon
https://www.researchgate.net/publication/351620685_Haemogregarine_of_Genus_Dactylosoma_Adeleorina_Dactylosomatidae_in_Species_of_Rhinella_Anura_Bufonidae_from_the_Brazilian_Amazon
For Plasmodium and Haemoproteus, scanning at 1000 x is necessary. Immersion oil is placed directly on the smear and the lens is lowered into the oil for scanning. (Be sure to use the immersion oil manufactured for the 1000x lens being used.) The oil will not harm the smear.
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