How can i excyst the eimeria sporulated oocyst and purify the sporozoits viable to infect in vivo or embryonated egg?

10 May 2022 1 940 Report

We want to produce oocyst of eimeria through embryonated egg passage. We have purified and sporulated the oocyst. Now I want to excyst the oocysts and purify the sporozoites. How can I store the sporozoites also to use for further study?

Tran Nguyen

Hi Asief,

i think you can find more information about how to purify and excyst Eimeria oocysts in this paper:” A modified method for purification of Eimeria tenella sporozoites”

Zaida Rentería-Solís et al 2020.

In our lab, we passage Eimeria oocysts in young chicks. You can easily and collect more oocysts.

People normally store the oocysts. You can think to store oocysts in K2Cr2O7 (can use up to 6 months). Prepare fresh sporozoites before experiments!

I hope it works for you!

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