Which tumor cell lines are being used for adoptive cell therapy?

Nowadays, engineered NK92 cells are being investigated and tested as an 'off the shelf' cell therapy. Why isn't there a T cell line investigated?

31 August 2020 1,530 3 View

Methods to attach glutathione agarose resin to glass slide?

Hello, I am looking to capture a GST tagged protein on a glass slide. I was wondering if anyone had any experiences with glutathoine agarose resin. I realize this is commonly used in affinity...

13 July 2020 1,773 3 View

What thresholds should I use for common variance/how do I interpret scores (i.e. good, fair, poor)?

I am trying to describe if common variance is good, fair or poor and need a reference or idea of what is acceptable. for example Comrey and Lee (1992) have suggested guidelines/cutoffs: .71...

29 May 2018 5,186 3 View

Reference protein to distinguish white and grey matter

13 July 2017 6,929 2 View

I would like to check for gender from my mRNA samples. Is it possible?

I have checked for gender using PCR after extracting the DNA. However now the samples were processed and lysed for RNAseq already. So is there a way to check for gender now?

15 November 2016 4,852 2 View

Project based learning ideas for first year biology majors - Animal Behavior and Conservation Biology

In search of assessment alternatives to exams as I now teaching online. Appreciate this supportive community and wanted to reach out. TIA.

01 January 1970 5,431 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View