Donovan Flumens

4 Questions 4 Answers 0 Followers

Questions related from Donovan Flumens

Does it matter where you place the DR sequence in you crRNA for Cas13 editing?

Do you place the Directed Repeat fixed sequence on the 5' end or 3' end of your spacer sequence when designing Cas13 crRNAs? Does it matter? Thank you

25 October 2024 1,192 2 View

How to you design zinc fingers?

Hi, How can I specifically design my zinc finger proteins so they bind to the sequence I want. Is it also possible to design RNA binding proteins or RNA-binding zinc fingers? Thank you

12 December 2023 7,278 3 View

What can you do with isolated RNA from immune/cancer cells beyond RNA-seq?

I am isolating RNA from immune cells and cancers cells and I am wondering how I can optimally us the RNA. I am looking for novel/forgotten ways to investigate isolated RNA, so beyond the single...

07 December 2023 5,295 0 View

Which tumor cell lines are being used for adoptive cell therapy?

Nowadays, engineered NK92 cells are being investigated and tested as an 'off the shelf' cell therapy. Why isn't there a T cell line investigated?

01 September 2020 1,742 3 View