How to validate the MSP Primers before ordering them and can we do the 5 gene methylation in a single tube? can anyone explain about how to carry out?

15 December 2023 0 1K Report

I Have designed the Primers for MS-PCR and how to validate them. can we do the 5 gene methylation in a single tube? can anyone explain how to carry it out? practical approaches for MS-PCR and technical guidelines if any?

Thank you,

Badges
Science method

More Sagar Nitturkar's questions See All

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

What is the difference between uv visible measurment and phocatalytic degradation measurment?

why uv is done and why photocatalytic measurement is done although both use uv radiation

09 June 2024 8,750 1 View

When can the fitting of the fluorescence anisotropy decays have the negative component ?

it would be helpful if you provide the reference to your answer

11 May 2024 5,989 4 View

Advanced research on ferrites ?

I just completed my M.Sc Physics while researching on ferrites in Photocatalytic property. Now i want to do My Phd does anyone suggest me any topic or any good university for doing my reserch

19 April 2024 3,772 2 View

Does the Primer3 design primers for the COBRA technique? and selecton of single Restriction enzyme for multiple genes?

Hello, RG Members, I have designed the COBRA primers using the UCSC genome browser for the promoter regions of genes of my intreset. are these primers reliable? I have tried the other softwares...

17 April 2024 7,882 0 View

Can any one tell me perfect mobile phase with ratio for paper and TLC of sugars?

I'm working on sugars present in honey sample.I tried paper and TLC with different mobile phases like Butanol:Glacial Acetic acid:Water and Butanol:Pyridine:Water but I'm not getting expected...

05 March 2024 9,609 1 View

Can any one tell me how to confirm methane gas production test?

I'm working on utilization of wastes for biogas production.I streak a loopful cowdung slurry collected from biogas plant on sodium thioglycolate media.I got some colonies on the media.I need to...

28 February 2024 1,877 4 View

HOw TO FIND THE RESTRICTION ENZYMES USING rs ID?

Can anyone knows about how to find the restriction sites using rs IDs. Are there any tools to find?

21 February 2024 2,794 0 View

Can anyone please tell me how to run TLC of amino acids using silica gel plate?

I'm trying to seprate amino acids using silica gel.When I spray ninhydrin solution on silica gel it get disturbed.Can anyone tell me how to observe separated amino acid on silica gel plate?

06 February 2024 8,366 2 View

Why my GST fusion protein is eluted out in wash buffer and flow theoigh compared to elution buffer?

Hello everyone, This is my first question and very curious to learn why my GST fused protein shows band in the cell Lysate flow through, lysis buffer, wash buffer, but absolutely no bands on the...

25 January 2024 7,470 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Why in 2D image created by discovery studio 2021, the halogens appear as alkyl group?

In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.

28 July 2024 734 0 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

How to Select the most suitable machine learning algorithm depending on the characteristics of the given dataset ?

I'm working on a project that involves analyzing a new dataset, and I'm at the stage of selecting the most appropriate machine learning algorithm. The dataset consists of both numerical and...

22 July 2024 6,097 7 View