Why do we need to give low pressure, high temperature and inert gas environment during synthesis of polymer?

I am working in synthesis of material.

14 December 2020 8,092 5 View

RS dataset of ice cover of Arctic Antarctic?

I am looking for datasets of ice cover of Arctic Antarctic. I want only India related dataset. If you have any source of dataset please share with me. It will be very helpful for my...

13 October 2020 7,137 1 View

How can I false color of SEM images (tiff format) ?

I have SEM image of cross section of multilayer thin film sample and need to represent the different color for different layer without losing its morphological information on the image. Is there...

08 October 2020 9,764 6 View

How to model deformable metal powder?? How to define gap between the particles?? Which modeling software can be used??

We need to model deformable metal powder. It will be further used in simulation process.

30 September 2020 9,329 7 View

Can we use homoeopathic high potency medicine for plant growth and development for their nutritional purpose?

The homoeopathic medicine consist minerals in it and the same nutrients are essential for the growth and development of the plant. So I asked myself whether the homoeopathic medicine useful for...

19 September 2020 8,339 10 View

When the Renewable Energy Sources integrated to the main grid, the with and without RE sources, how the system characteristics performance?

While incorporating the DER to the conventional grid, then what will be the effect of system stability and frequency

07 September 2020 2,386 7 View

Can anybody suggest how to prepare monosilicic acid from any source of silicon?

Monosilicic acid is the available form in which plant can take silicon and this is not a stable form. That's why I want to know how to prepare that and how to estimate that. Kindly give your inputs.

01 July 2020 6,226 3 View

Should we adopt the latest tools of Precision agriculture immediately? If not now, then when?

Precision agriculture (PA), in India, is synonymous to Remote sensing, GIS, GPS, VRA and VRT. These are old technologies and during last 3-4 decades enough research has been carried out. But the...

11 March 2020 8,581 15 View

Can we differentiate aerobic microbes from anaerobic microbes while isolation?

Just wants to know whether we can differentiate aerobic and anaerobic bacteria from same isolation method or we have to follow another method of estimation for anaerobic bacteria.

07 February 2020 7,351 5 View

How long the traditional nutrient management trials in crops will be continued with graded levels of fertilizer?

During last 5-6 decades we have generated enough data from traditional experiments on nutrient management with graded levels of fertilizers in almost all systems and situations. Research on...

04 February 2020 7,451 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

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02 March 2021 932 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View