Transfecting HEK293T cells with in vitro transcribed (IVT) sgRNA and a plasmid carrying a dCas9 backbone requires an efficient transfection reagent to ensure successful delivery of the genetic material into the cells. The choice of transfection reagent can significantly impact transfection efficiency and cell viability. Lipid-based transfection reagents are commonly used for this purpose. One of the most popular choices is Lipofectamine 2000, but there are other alternatives as well. Here are a few options:

When choosing a transfection reagent, it's important to consider the specific requirements of your experiment, such as the cell type, the amount of genetic material, and the presence of any additives or modifications in your sgRNA or Cas9 plasmid. Additionally, perform transfection optimization experiments to determine the best conditions for your particular setup, as the ideal reagent and protocol may vary from one lab or experiment to another. Always follow the manufacturer's guidelines and recommended protocols for the transfection reagent you select.

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