Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

What should we do if?

if we do not get appropriate GC content and melting temperature while designing primers for full gene amplification? i have a nucleotide sequence of sars cov2 surface glyco-protein of which i have...

20 July 2020 8,085 2 View

What should be the value for dissipated energy factor for the varification of the experminetal data for a concrete beam?

What should be the value for dissipated energy factor for the varification of the experminetal data for a concrete beam? What exactly should i choose from the experimental data to numerical...

16 October 2019 4,417 2 View

Can any one share a reliable transfer function model of wind and/or solar energy generation sources for load frequency control (secondary control)?

We have transfer functions for other generation sources like non-reheat steam turbine (1/1+st). I am looking for same kind of transfer function for Renewable sources.

21 November 2018 7,457 3 View

How to stabilize the pH of NaNO3 solution?

I am doing a soil column experiment, using quartz sand as a transport medium. We are using 0.001 M NaNO3 as a background solution. Before injection to soil column the pH was adjusted to 7.5 with...

12 August 2018 9,630 2 View

Power Control With Z-Source Converter Based Unified Power Flow Controller UPFC?

I want to simulate on MATLAB SIMULINK Three Phase Z source based UPFC for IEEE-30 Bus bar standard with line to line ground fault. any one done work on ZSI UPFC , kindly guide . regards

06 April 2018 3,504 1 View

I want some literature regarding Phosphate nanoparticles, their synthesis and mainly about their transport in porous media?

Phosphate nanoparticles

05 February 2018 4,382 3 View

Anyone can suggest me an effective quantitative instrument for assessing the performance of software packages among oil and gas industries?

28 May 2016 1,570 5 View

How can I compare crash characteristics between two locations?

I have to compare the crash characteristics two roads at two different geographical conditions, A and B. A have their own segments A1,A2,A3 and A4. B also have their own segments B1,B2,B3,B4 and...

08 December 2014 382 22 View

Any published article on this work pls?

I want to see how it will help in optimization of a harvesting machine

01 January 1970 8,436 2 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View