i am making spore stock of streptomyces, that i isolated from different regions of Nepal. Use of charcoal and glass wool for making glycerol stock is the best method that i got from various papers, so .please provide me the details
G. P. Robertson. 1999.Standard Soil Methods for Long-term Ecological Research Oxford University Press,Pp.462. ISBN0195120833, 9780195120837
https://www.cdc.gov/niosh/pdfs/76-139e.pdf
2005.The Code of Federal Regulations of the United States of America United States. Division of the Federal Register, United States. Federal Register Division, United States. Office of the Federal Register https://books.google.com/books?id=kRuHAAAAMAAJ&dq=charcoal+and+glass+wool+procedure&hl=ar&source=gbs_navlinks_s