How to trouble shoot this γ-H2AX detection by WB?

Heba Salah @Heba-Salah-20

17 March 2025 0 7K Report

The conditions I used are:

1- sonication: AMPL:20%, 30 sec (10 Sec pulses).

2- Boil at 95 for 5 Min

3- Run in SDS at 95 V for 90 min

4- 4-20% gel

What may contribute to this waving and how to fix it?

Thanks in Advance.

Heba

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