How to treat DNA abnormality, the CT Scan of Brain observed the Mega magna cisterna detected ?

14 December 2017 0 2K Report

The chronic pain above the Cerebellum, the treatment of migraine from past 2 year, but no effect on symptoms.

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How to compile a udf in Fluent to import hourly weather data and update the boundary condition based on it ?

Hello all, I've compiled a udf which reads velocity data with corresponding time stamp from a .csv file using DEFINE_ON_DEMAND macro. Then, I use DEFINE_PROFILE to define an inlet velocity profile...

14 June 2024 6,480 0 View

Does autoclaving reduce the concentration of heavy metals in sewage wastewater?

I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...

10 June 2024 2,579 4 View

How to start learning VIC-4.2 Land surface model? Can anybody give me some suggestions?

I have installed a linux system for the same purpose and downloaded the VIC-4.2.d model. While looking for any tutorial series and manual for use and operation of VIC is not found. I would be very...

29 April 2024 9,097 1 View

How to write an article ? Give writing method step by step ?

13 April 2024 1,479 3 View

Can anyone help me calculate Cremer & Pople puckering parameters from a PDB or XYZ file using PLATON software, online, or any other simple method?

Can anyone help me calculate Cremer & Pople puckering parameters from a PDB or XYZ (or Cif) file using PLATON software, online, or any other simple method? (free downloadable windows supported...

02 March 2024 8,945 1 View

I have been facing problem of cell layer coming off during virus titer especially while incubating cells with virus for 1 hour for attachments?

I am been using VEROTMPRSS2 cells and incubating cells with 200ul of virus dilution for one hour. Cell layers are coming off after 30 min incubation. What might be the best solution to keep cell...

07 November 2023 9,802 2 View

How to analyse Anisotropic hyperfine parameters?

I calculated anisotropic hyperfine parameter using gaussian 9 and got values as follows: For example: Atoms MegaHertz C(13) Baa -6.995 Bbb...

05 November 2023 8,357 0 View

How can I calculate phase angle at the output ports in transmission grating structure?

I want to measure the phase angle in comsol.

06 October 2023 8,185 2 View

How to see phosphorylation of p-IRF3 protein in mouse macrophages cell line? I used HMW poly I:C(10ug/ml) to stimulate the cells but no results?

I collected samples at 30min,1 hrs,2 hrs,6hrs,,12hrs and 24 hrs. But still couldn't detect phosphorylation. If anyone has done it, could you please suggest me better stimulation and collection time.

28 September 2023 5,060 2 View

Substitution of La in ZnO lattice use to cause xrd peak shift towards which direction?

Substituting La in ZnO lattice shift xrd peaks towards right or left - higher angle shift or lower angle shift.

12 August 2023 3,547 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

In the situation below, can I apply braun and Clark thematic analysis?

I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).

04 August 2024 8,798 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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01 August 2024 2,079 0 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How are aPTT and PT values reduced after hemodialysis compared to before dialysis value in patients with chronic kidney disease?

I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...

30 July 2024 3,467 0 View