What happens in post-secondary education when instructors don't use traditional grades?

I am looking for empirical studies that examine the use of non-traditional grading in higher education. This means NOT using A-F grades during a course (even if, at the conclusion of the course,...

09 June 2020 1,519 6 View

Vimentin Western Blot: No consistency of the change in vimentin expression level of A549 cell line. What can I do?

I am working on Epithelial to Mesenchymal Transition (EMT) in A549 cell line by treating positive inducers such as EGF to up-regulate the mesenchymal markers like vimentin. I'm doing western blot...

22 May 2019 3,053 1 View

What is the CSL boundary in martensitic steel?

I know that the CSL boundaries in fcc and bcc structure are different. For example, Sigma 3 in fcc is 60degree, but that in bcc is 70.5. However, I've seen analyses of the...

06 June 2017 7,967 3 View

Are there any BCC metals that showing no DBTT phenomenon?

Generally, BCC metals show a Ductile-Brittle Transition phenomenon as decreasing temperature. A main cause is that the temperature dependency of yield strength is large, thus the yield strength...

04 June 2017 7,891 2 View

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02 March 2021 3,060 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View