Claira Sohn

5 Questions 3 Answers 0 Followers

Questions related from Claira Sohn

How to treat cells with Kinase Inhibitor to analyze if extra IP bands are due to phosphorylation?

Hey everyone, I bought this kinase inhibitor (Sigma 20-116). It did not come with any instructions/protocols in terms of treating cells with it. I plan to use the cell lysate for an IP. When...

09 February 2024 7,479 0 View

Can I stain DAPI or phalloidin when using FRET?

Hey! I have a FRET system between the inner and outer nuclear membrane, and I was wondering if I stained with DAPI or any other fluorescent antibody if that would mess up my FRET readout? As of...

07 December 2021 10,022 2 View

Has anyone used the Corning Matrigel with a Be(2)-c cell line?

Hello, I am currently trying to study cellular stiffness in the Be(2)-c line and I want to use collagen stiffening matrix by corning. Has anyone had success doing this? If so, do you have an...

19 October 2021 4,543 0 View

Why is my FRET donor decreasing during acceptor photo bleach?

I am using the acceptor photo bleach method with Venus and mTFP1. Recently, I have applied this sensor to a model and I was expecting to see an increase in FRET due to less tension, however they...

06 September 2021 5,912 1 View

I am analyzing Acceptor photo bleach FRET data, but when I use ImageJ "image calculator" my donor channel is too weak. Any suggestions?

Hello! I am currently working on FRET using acceptor photobleaching. I have obtained images from a Zeiss 880 confocal. Using the ROI feature, I am able to calculate a 15-30% increase in the donor...

22 April 2021 9,479 4 View