Hello,
I am currently trying to study cellular stiffness in the Be(2)-c line and I want to use collagen stiffening matrix by corning. Has anyone had success doing this? If so, do you have an available protocol?
Thank you in advanced.
Hey everyone, I bought this kinase inhibitor (Sigma 20-116). It did not come with any instructions/protocols in terms of treating cells with it. I plan to use the cell lysate for an IP. When...
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I would like to check the possibility of implementation of BO using C language. The implementation target is MCU like Tricore or further. Is this possible? Moreover, is there anyone know where...
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Hey! I have a FRET system between the inner and outer nuclear membrane, and I was wondering if I stained with DAPI or any other fluorescent antibody if that would mess up my FRET readout? As of...
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I developed automatic tuner using Bayesian Optimization. The plant system is real vehicle. Furthermore, there is no stability problem, because this system is a kind of semi-active control...
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I am using the acceptor photo bleach method with Venus and mTFP1. Recently, I have applied this sensor to a model and I was expecting to see an increase in FRET due to less tension, however they...
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Hello! I am currently working on FRET using acceptor photobleaching. I have obtained images from a Zeiss 880 confocal. Using the ROI feature, I am able to calculate a 15-30% increase in the donor...
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I am looking for empirical studies that examine the use of non-traditional grading in higher education. This means NOT using A-F grades during a course (even if, at the conclusion of the course,...
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I am working on Epithelial to Mesenchymal Transition (EMT) in A549 cell line by treating positive inducers such as EGF to up-regulate the mesenchymal markers like vimentin. I'm doing western blot...
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I know that the CSL boundaries in fcc and bcc structure are different. For example, Sigma 3 in fcc is 60degree, but that in bcc is 70.5. However, I've seen analyses of the...
06 June 2017 8,103 3 View
Generally, BCC metals show a Ductile-Brittle Transition phenomenon as decreasing temperature. A main cause is that the temperature dependency of yield strength is large, thus the yield strength...
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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