Hi,
I'm trying to differentiate iPSCs to Immune cells and looking for plates with low-attachment. I came across this reagent "Anti-Adherence Rinsing Solution" by stemcell technologies which is a surfactant solution for pre-treating cultureware to reduce surface tension and prevent cell adhesion.
But, my question is: can I use this solution on any TC treated plates or should it be only from aggrewell brand? Has anyone used it before?
https://www.stemcell.com/products/aggrewell-rinsing-solution.html Catalog #07010.
Thanks.
Vertica
Hi Vertica,
My name is Audrey and I am a Product Manager at STEMCELL Technologies.
The Anti-Adherence Rinsing Solution is effective when applied to any plate type, not just AggreWell™ plates. However, its performance is not optimal on tissue culture-treated plates and you may see failures after a couple of days.
Our Product and Scientific Support Team would be happy to work with you directly and troubleshoot this issue—you can email them at [email protected].
I hope this helps!
Kind regards,
Audrey
Hi Vertica Agnihotri
Were you able to try this?
I am performing a phagocytosis assay on mouse peritoneal cavity cells and detecting them using flow cytometry. Since the cells remain on the culture plate for 5 hours during the assay, they become strongly adherent. Scraping them in 2 mM EDTA for flow cytometry is causing significant damage—I see most of the cells appearing as debris in the FSC/SSC plot. I'm using non–tissue culture–treated plates, but the cells are still strongly adherent. So, I'm considering treating the plates with anti-adherent solutions to reduce cell adherence. so, wondering whether you were able to try this?
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