Hello everyone and Very Happy New to all.
Could any body please share the format of nexus file to build a phylogenetic tree using 16S rRNA for Mr. Bayes. and how can we select the model for Mr.Bayes to run the tree?
Thank you very much!!
Hello, It is a very general question. I am doing pigment extraction (phycocyanin and phycoerythrin) from cyanobcateria using liquid nitrogen freeze and thaw method. The method i am using does not...
11 December 2016 7,694 4 View
I did DNA extraction of cyanobacteria. Up until the very end it seemed to be working fine. The Chloroform/isoamly alcohol (24:1) steps all formed a separate layer when introduced and seemed to be...
10 November 2016 7,359 7 View
Hi, I am doing molecular identification of my samples (cyanobacteria) using ITS and 16s gene sequences. For ITS region, once i BLAST my sequences against the NCBI data it just showed 94%...
06 July 2016 9,951 4 View
Hi, I am doing cyanobacteria DNA extraction. I have used many preassembled kits for DNA extraction but could not get good purity. I tried ethanol purifictaion but i lost 60% of DNA. Pls suggest...
03 April 2016 9,212 5 View
Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...
08 August 2024 1,491 0 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
My case has been under treatment now for 3 years with little response. Now considering euthanasia. Last ditch is Cisplatin for dilution in iv infusion (100ml/ml in saline and mannitol). What can I...
05 August 2024 1,835 0 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...
29 July 2024 9,572 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Please, what is the memory consumption of the Matlab function quad tree decomposition procedure [S = qtdecomp(I)] with respect to the input set I?
27 July 2024 5,455 2 View