Zoya Khan

5 Questions 9 Answers 0 Followers

Questions related from Zoya Khan

How to make a nexus file and how to select the model in Mr.Bayes?

Hello everyone and Very Happy New to all. Could any body please share the format of nexus file to build a phylogenetic tree using 16S rRNA for Mr. Bayes. and how can we select the model for...

12 December 2016 7,013 0 View

How to thaw samples?

Hello, It is a very general question. I am doing pigment extraction (phycocyanin and phycoerythrin) from cyanobcateria using liquid nitrogen freeze and thaw method. The method i am using does not...

12 December 2016 7,566 4 View

Why my DNA got salt pellet?

I did DNA extraction of cyanobacteria. Up until the very end it seemed to be working fine. The Chloroform/isoamly alcohol (24:1) steps all formed a separate layer when introduced and seemed to be...

11 November 2016 7,268 7 View

How many percent similarity is acceptable with NCBI Blast search in case of ITS region?

Hi, I am doing molecular identification of my samples (cyanobacteria) using ITS and 16s gene sequences. For ITS region, once i BLAST my sequences against the NCBI data it just showed 94%...

07 July 2016 9,903 4 View

Please suggest protocols for good DNA purity?

Hi, I am doing cyanobacteria DNA extraction. I have used many preassembled kits for DNA extraction but could not get good purity. I tried ethanol purifictaion but i lost 60% of DNA. Pls suggest...

04 April 2016 9,164 5 View