it is better to attach your microbeads to glass. indeed, it depends on functional groups of microbeads you have. Attaching samples is usually used to measure force curve, e.g. in case of bacteria, cell, algae, etc.

Wisnu Sudjarwo, Can you please suggest any method by which polyacrylamide microbead can be attached?

Hassan Liaquat You can use Bind-Silane (please refer this website: https://www.abbexa.com/binding-silane?srsltid=AfmBOoo7QhIYs83OJvS88xfWgRPJxwZPg04aPmezQ5fGN8zhQE9-vUj9)

There are several papers used Bind-Silane product to attach acrylamide beads. Please check here:

https://pubmed.ncbi.nlm.nih.gov/?term=Bind-Silane

Or, you can use this method:

1. silanization of glass using APTES (to introduce amine groups).

2. introducing aldehyde group after silanization using glutaraldehyde

3. since acrylamide has amine groups, they can react with aldehyde group. so just add the beads.

4. You can change glutaraldehyde with DSS (disuccinimyl suberate)

this above method is publicly known.

Wisnu Sudjarwo Thanks Wisnu, i will give it a try. beads are in the form of suspension, as i cant make sure to remove all the liquid content, so i wil putting beads onto the slide in the form of tiny droplet, will it still work?

No problems. I think it will work.

Dear Hassan, could you dry your sample? If so, you can use double-sided tape to secure your particles. Additionally, you can use a compressed air jet for a few seconds on your sample to ensure they are firmly fixed.

Hi, Monique Lunardi , I can't dry them, as they will dehydrate rapidly, which will alter their mechanical properties.

But I will try compressed air, thanks for your comment.

I hope this helps