We are trying to take pictures of conidiophores of filamentous fungi (ascomycetes etc.) under a microscope (X20, X40, X66). We try to get good pictures of conidiophores bearing some conidia. But often conidia scatters and we see only conidiophores without conidia.
Is there any good protocol to follow? Thank you.
Hi Saito
Use adhesive and transparent tape and put it in contact with the colony, in general, many of the conidiophores remain in their original form.
Thank you for your reply. I already tried tape-method and I know it works for some fungi but it doesn't for others. And I was wondering if there is some kind of standard protocol out there that I didn't know existed.
Kouadri Mohamed El Amine Thank you for your reply. Yes. I tried slide culture method by putting a tiny agar media on the slide with spores, and let it grow for a while. I also prepare another version of this by putting the slide on a heat block around 60°C for a while to melt agar thereby flatten the slide. This works good sometimes.
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