I want to tag the polymer capped nano particles with a fluorescent dye. Most of the method that I have referred do not work. They are simply not reproducible even though they were published in reputed journals.
Please suggest me a method which truly works and also applicable for in vivo experiments.
Thanks!!!
You can look up fluorescent labelling of proteins, nucleic acids etc and implement the same chemistry here. For example, Companies like SigmaAldrich, Thermofisher etc have fluorescent dye protein labeling kits
Thank you for the answer, Dr. Ravi. But NPs labelling differs from labelling a peptide or nucleic acid. I had experienced.
Ultimately it depends on the functional groups and if the functional groups are same then it is the same chemical reaction. You may have to be careful with the reaction conditions so as to not cause aggregation.
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