How to study gene promoter using CRISPR CAS9 in vivo

More Johan Ariff Mohtar's questions See All

Please suggest literature reviews on public sector innovation capabilities?

I am looking for literature reviews focusing on public sector organizations capabilities for participating in and coordinating innovation together with both private and non-governmental organizations

09 June 2024 7,818 5 View

Is E = mc^2 the potential energy of a mass m at the cumulative gravitational potential originating from remote masses of the universe?

The potential energy of a 1 kg mass due to the sun's gravitational potential at the earth position is about 109 J (1 GWs). The cumulative gravitational potential of all masses within the visible...

29 December 2023 1,242 15 View

Are there any scales for body image satisfaction with interpretation, scoring, and questions that I can use?

I'm currently researching the levels of body image satisfaction of underweight and overweight adolescents. However, I'm having trouble finding scales with the full interpretation, specific scoring...

24 October 2023 8,520 3 View

What scales can I use to measure an adolescent's satisfaction with its body image and to measure an adolescent's social phobia levels?

I am currently researching the levels of body image satisfaction and levels of social phobia of underweight and overweight adolescents. I am having trouble finding the scales to measure these...

15 October 2023 656 3 View

How do I compute range in a likert scale with 0 to 4?

If you use a likert scale with range "never" as 0, 1 is "one to two times", 2 is "sometimes", 3 "often" and 4 "most of the time". How to correctly compute the range? Is the lowest value counted?...

29 September 2023 7,535 3 View

How to correctly describe and model the diffuse reflectance measured from a set of optical fibers in skin?

There are different approaches to diffuse reflectance modeling, most of them based on the solution of the radiative transport equation by the diffusion-based approximation. However, a set of fiber...

30 July 2023 7,616 1 View

Fractography - how do I clean a charred fracture surface?

We have a failed steel pipe that after failure is heavily charred which covers the fracture surface so that we cannot do a proper failure mode analysis The carbon does not come off using US or...

16 May 2023 968 1 View

How to troubleshoot failed salt test in passive conjugation of AuNP and antigen?

Dear colleagues and experts, We were trying to conjugate an antigen with 48kDa with 40nm AuNPs. We tried using 20ug of antigen (diluted in 20mM PB, 150mM NaCl,1‰P300 buffer) + 250ul of AuNPs OD20...

11 May 2023 3,530 3 View

Problem with printing. How can I revise one of my papers published by Research Gate?

Some of the equations in one of my papers did not print right. How can I fix them?

01 May 2023 9,447 0 View

How do I update one of my published papers?

I noticed that the text published on Research Gate does not match my original.

01 May 2023 8,347 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Why knockouts for same genes from two different gRNA are showing different result?

Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.

25 July 2024 8,313 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Santosh Shelke

Please find the article of the fallowing authors. This will help you in that regards.

Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nature biotechnology. 2015 Jan 1;33(1):102-6.

Johan Ariff Mohtar

Thank you mr Santosh Shelke for the infor