Dear all,
I am just trying to do an overall stimulation of PBMCs. Anti CD28/CD3 to stimulate T cells, but how can I stimulate the innate response? Some TLR agonist should be fine? TLR agonist can alter the natural T cell responses? Can I ask for some papers in which I can check this, please?
Also, if I am checking CD28 marker by flow cytometry, can the addition of anti-CD28 alter the detection of real levels of CD28 by flow cytometry?
Many thanks,
Julen.
Generally, we use LPS to stimulate TLR of PBMC. LPS (100 ng/mL) in 5,0x10^5 cells by 6-12h works very well.
Hi Julien!depends which TLR you want to activate to induce inflammation, TLR2 or TLR4, you have specific agonist like PAM3csk4 for TLR2 and LPS or heat-killed listeria monocytogenes or LPS as mentioned above for TLR4