5 Questions 9 Answers 0 Followers
Questions related from Julen Tomás-Cortázar
Hello, Can Alexa Fluor 488 Polyclonal Antibody be used to immunoprecipitate proteins? Has someone tried it or has experience with this? Many thanks, Julen.
26 May 2023 6,285 1 View
Does anyone know about a reliable CD8 T cell epitope prediction tool for protein-based antigens? Many thanks in advance! Julen
03 February 2023 7,863 1 View
Dear all, I am just trying to do an overall stimulation of PBMCs. Anti CD28/CD3 to stimulate T cells, but how can I stimulate the innate response? Some TLR agonist should be fine? TLR agonist can...
30 March 2022 9,977 4 View
Hello, I washed the column with 0.5M NaOH and then 20% ethanol, then equilibrated with the running buffer, then injected my sample and did the purification. After purification, I washed the column...
08 September 2021 8,039 4 View
Hi all, I want to remove the endotoxins from my protein sample, since they could overestimate the immune response of the antigen. I measured the endotoxin level in my sample after gel filtration,...
07 January 2019 2,314 7 View
