There are various indigenous species that are available only in India. So how biodiversity and taxonomists, ecologists make this claim. At least certain terrestrial animals that are endemic to...
06 March 2024 8,920 1 View
I have some queries regarding dispersion of ZnO NPs by ultrasonication and also solvents used for dispersion, like DMSO, ethanol, etc., 1. Dispersion by ultrasonication Upon synthesizing zinc...
28 November 2023 5,347 1 View
For findining minimum inhibitory concentratio(MIC) and Minimum bactericidal concentration(MBC) of green synthesized zno nps, I tried to dissolve the zno nps with different concentrations (2.5,...
19 October 2023 9,199 8 View
I want to practically simulate the fixed support for experimental modal analysis. But no reference has been found regarding, what should be the frequency of the support compared to that of...
06 June 2021 275 5 View
I would like to know the exact number like it can be reused for 5 or 10 times etc.
26 April 2020 1,695 2 View
Please gave me suggestions such as which laser to use for task i & ii. Whether I need to use the same system for both the tasks or not. If possible please provide details regarding beam...
10 April 2020 7,960 0 View
I need to compare the sample for porosity determination by using x-ray CT and optical microscopy. All views and suggestions are welcome.
27 March 2020 6,610 3 View
I would like to know about the coating possibilities of PTFE on PP or any other fluorinated polymer on PP to decrease surface energy. All suggestions are welcome.
27 March 2020 9,809 3 View
1. which is the most recent and effective method for removal of fungal contamination inside the clean room? Is it Fumigation or Fogging? 2. I am getting the same fungal growth inside clean room...
26 June 2019 6,204 4 View
I have a model which depicts that when velocity of the water increases at the inlet, temperature measured across the outlet decreases. The results are shown below INLET VELOCITY(m/s)...
05 April 2016 2,089 14 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
please suggest the research paper related to the instrument is used in it.
16 July 2024 8,453 1 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...
16 July 2024 7,937 1 View