10 March 2019 1 4K Report

If we have amino acid composition of the material, how can we optimise ingredients to get essential amino acid balance.

More Niharika Sachdev's questions See All
How can I make the CST simulation run faster?

I am trying to simulate a metasurface of 24x24 elements, but even with a GPU of 48GB it takes very long to simulate. I want to obtain the field along the defined curve.

10 June 2024 3,577 7 View

Why is my protein concentration increasing after storage at -20°C?

I have a few proteins that I produce recombinantly in E. coli cells. All of them are disordered proteins and are stored in the presence of 4mM DTT at -20°C before use. One of them also contains...

28 November 2023 2,682 2 View

Why do A431 cells form clumps in propidium iodide staining solution?

I've been trying to study cell cycle using PI (propidium iodide + Triton-X + sodium citrate)b staining solution. I trypsinize my cells (A431: skin carcinoma), fix them using 100% methanol and...

20 November 2023 2,443 3 View

Not getting any protein expression. How to tackle the issue?

I am trying to express a novel GlcNAcT enzyme in BL21(DE3). I have successfully cloned it in pET28a vector and confirmed it through sequencing. I tried following previous protocols for GlcNAcT...

19 July 2023 6,064 2 View

Is there any public database that has both chest x-rays and CT Chest available?

The NIH has separate databases for chest x-rays and CT Chest but I am looking for any public one that has both available to compare some variables between both.

12 July 2023 3,530 2 View

How to obtain sensitivity from cst?

I am working on obtaining sensitivity of microstrip patch in cst but I cannot seem to understand how to obtain the plots in the paper ( page 6044/fig 8). I manually derived the formula for...

27 February 2023 5,663 0 View

How to conduct qualitative detection of sialic acid using resorcinol method on TLC plates made with silica gel??

I am qualitatively trying to determine the sialic acid using resorcinol on TLC plates. Can someone help me with the process?

26 January 2023 7,825 0 View

How do I grow WI-38 (human normal lung) cell line?

I have WI-38 cells. Although the cells look healthy, they are growing very slow,. I have increased the serum (FBS) from 10 to 15%, cultured in high glucose and high glutamate media, yet it is not...

05 December 2022 6,450 2 View

What is the role of 8M urea in protein purification buffer besides solubilization?

Hello, I am trying to express and purify recombinant nuclear pore proteins in E. coli cells. All the published protocols I have found use 8M urea in the lysis buffer and subsequently during...

19 October 2022 4,623 5 View

How to get sample wise allele frequency in a VCF file?

Given a multi sample VCF file how to obtain AF for each sample in the file

17 October 2022 2,525 1 View

Similar questions and discussions
How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How can AI-driven personalized learning balance individual instruction with fostering social and collaborative skills?

This question tackles the complex issue of ensuring that while AI can tailor educational experiences to individual needs, it doesn't neglect the equally important aspect of fostering social...

02 August 2024 4,751 3 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

What are the steps of formulating a herbal extract into a sheep/goat drench?

I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...

30 July 2024 9,806 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

How Can I Apply Hashin Damage Properties to Solid Elements in Abaqus Without Using VUMAT?

Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...

28 July 2024 8,169 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close