i am going to experiment with immunohistochemistry in relation to salmonella and macrophage.
by the way, i have no experience with IHC.
so, i write such a this short letter for you in order to give your hands.
first of all, the biggest problem is that i do not know weather macrophage inner proportion is possible to be stained by any other stain buffer or not.
if there are people who experience with IHC, then give me a good way and discussion.
i will wait for your help.
Macrophages are antigen presenting cells and getting a specific marker to an antigen presenting cell can be a difficult task as they express different markers. Nonetheless, several reports hint to using F4/80 as a specific marker for mouse macrophages and I am currently using this marker both to immunophenotype tumor infiltrating macrophages using FACS and to detect their presence in tumor tissues using IHC. To come back to your question of how to do the immunohistochemistry, I think that will depend on the source of your reagents particularly the primary antibody and the type of detection (biotin, HRP, ...) as the protocol can be different accordingly which can be learnt from the data sheet of the antibody.
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