Tareg Omer Mohammed

4 Questions 14 Answers 0 Followers

Questions related from Tareg Omer Mohammed

Can anyone help with IHC (mouse tumor tissue) using F4/80 ( CI:A3-1)?

Greetings, An established in-house protocol has so far proven unsuccessful to stain tumor samples for macrophages using F4/80 (CI:A3-1) : sc-59171. It would be highly appreciated if someone can...

15 June 2016 9,434 2 View

How to make tumor size measurements more objective?

Greetings, These days working on s.c. tumors implanted on mice and felt like the process of tumor size measurement has much subjective element in it. I use calipers to measure the longest side of...

31 May 2016 2,241 4 View

Does it have any unfavorable effect to use PBS, 1%BSA as a wash buffer for a luminex based immunoassay?

I was wondering if it would create any problem using a PBS, 1%BSA buffer as a wash buffer for a luminex based immunoassay. So far I have been using a wash buffer that comes as part of a Bio-Plex...

08 September 2014 8,881 5 View

Is it okay to use the same capture-detection antibody pair that is used for the assay to determine coupling efficency of luminex magnetic beads?

I am working on developing a luminex sandwich immunoassay and discovered that the literature (luminex xMAPCookBook) recommends carrying out a coupling efficiency test. I used the same antibody...

22 August 2014 7,984 3 View