Please share the protocol
I wonder why would you stain nuclei if you are thinking of RNAseq?
Just to confirm before sending it for library prep.
spectrophotometric determination of iron and copper
04 June 2024 1,720 0 View
Hello Seniors, I need some help, I want to find out thermoelectric properties of 2D materials by VASP software can you tell me All steps and important INCAR tags.?
02 June 2024 7,093 1 View
The mankind is on the edge of resistance to peace. If you view news you will find that every day there are portions of the world where wars are happening and many innocent people are being killed....
26 May 2024 4,633 3 View
i am working in a diagnostic lab and very confusing about the calibrator, control and standard that we are using in our lab for quality assuarance of diagnosis of disease
07 April 2024 5,763 2 View
If I have to make the cloning strategy, what should be my workflow to do this. I got two problems, one to select suitable expression vector from pCAMBIA1301, and pCAMBIA1302. As 1301 has GUS...
17 February 2024 8,748 2 View
If the gene sequence for a specific gene (e.g., Asparaginase) is not found in the NCBI database, how can explore alternative strategies to identify and obtain the gene sequence in the organism of...
02 February 2024 1,910 3 View
Outliers detection criteria in a data set.
04 January 2024 2,806 1 View
blood tranfusion
17 December 2023 4,126 2 View
I have been assigned to write the introduction of research proposal?
24 November 2023 5,473 3 View
Hello seniors Im new, I want to make calculations of Electronic, magnetic, optical and thermoelectric properties 2D materials by VASP. please help me. Which properties I need to find first and...
26 September 2023 4,508 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
