Please share the protocol
I wonder why would you stain nuclei if you are thinking of RNAseq?
Just to confirm before sending it for library prep.
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
For example Decision Tree, Ant Colony Optimization etc.
26 December 2020 6,804 3 View
Case: Hg2+ is adsorbed on a titanium carbide and 0.1 M HCl/HNO3 is used for the desorption of Hg2+. During the reaction, the solution turns into a pale yellow color. I want to understand what...
16 November 2020 5,770 3 View
Is there any way to connect excel data sheets with aspen plus as inputs to perform parametric analysis?? I want to change input parameters for a certain range to see system performance
28 October 2020 2,174 3 View
Swelling behaviour of PMMA with GO contents 0.1%, 0.2,0.3,0.5?
02 October 2020 2,655 3 View
Any useful software for applying neural networks in power systems? I know MATLAB has a NN toolbox but besides that, is there any other useful software available?
20 April 2020 9,663 16 View
Any suggestions on where to begin learning neural networks for beginners? Some advice on useful resources , books, papers, etc will be appreciated. Thanks
18 April 2020 8,153 13 View
Any suitable index for describing power system frequency stability under a three phase fault?
01 April 2020 9,204 12 View
what is DPL command to find maximum value in a graph drawn in Digsilent Power Factory?
28 March 2020 5,674 3 View
I am having some trouble understanding the regression output. The author used an underlying statement to interpret his results. The baseline regression model is Cash holding = alpha +...
24 March 2020 1,784 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...
24 February 2021 4,618 4 View
