If we found Gram Neg, bacilli ... and not E. coli... contaminated in cell culture??? I dont have time enough for identification so What is the most suitable antibiotic that we will use? Not Pen and Strep.
Thank you
Surachai
Why not Pen/Strep?
Because they resist to Pen/Step, which normal antibacteria added in culture media
won't it do by simply streaking the suspected sample in EMB agar?
How to mix plant oil to culture media?
19 September 2012 9,952 8 View
Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.
26 July 2024 2,702 1 View
In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?
20 July 2024 4,020 0 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View
I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...
07 July 2024 8,671 6 View
Performance Standards for Antimicrobial Disk Susceptibility Tests, 14th Edition , please shre this file if anyone have.
29 June 2024 6,929 1 View
I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...
10 June 2024 9,564 6 View
Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?
10 June 2024 9,739 0 View
Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...
08 June 2024 2,285 6 View
So I made a 100 mg/ml solution of Ampicillin and in order to solubilize the powder I used 1 M NaOH. I noticed the solution turns yellow shortly after dissolving and I was wondering if some...
05 June 2024 7,170 0 View
The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.
01 June 2024 7,419 0 View