Let's assume that when the cell viability experiment was conducted using animal cells other than HeLa cells, the absorbance of the negative control (cells not treated with Doxorubicin) showed almost no difference from the value of the blank, and cell viability was not well measured. . In this case, please describe a strategy to solve the problem.
Hi Jules Lee,
There are several factors that may impact the cell viability in this case.
1st case, Assuming you are using the same cell line, then it may due to that the dose assigned is not strong enough to inhibit the cell. In this case, a drug dosing curve will assist in finding the IC50 of the cell line.
2nd case, Assuming you are using different cell line, and only some are resistance to the same dosage of the drugs, then it indicate these cell lines tested is resistance to the drug inhibition. If not refer to 3rd case.
3rd case, the doxorubicin may be degraded. It may sound weird, but drug may be degraded, regarding how the storage are. In this case, an pre-test with a positive internal control (with cell that expected sensitive to doxorubicin) and negative internal control (with cell that expected resistance to doxorubicin) will give the answer. Also similar case can also occured in the cell viability kit used in this study.
4th case, the incompatible of the cell viability assay used. It is very rare for this case, but sometime, the cell line may not respond to the cell viability assay used. In this case, you may need to swap the viability assay, for instance alammar blue assay to cell titre Glo etc.
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