Multi band on western blot for glucocorticoid receptor. Which band(s) should I select for quantitative study?

Hello I can see different bands for phosphorylated glucocorticoid receptor protein on the blot membrane. I get confused which band(s) should I select? Can I select the whole areas where the bands...

17 August 2020 6,771 8 View

Is it possible to have machine learning techniques in realtime systems?

Machine Learning (ML) is used to exploit some kind of predictability in the system, while in realtime systems you have to know the exact values and their isn't any place for probabilities. So, do...

07 March 2019 2,027 3 View

What is the best method to isolate amygdala from the fresh brain in rats?

I would like to isolate hipocampus and amygdala in rat. what is the best way that I can do it with the least tissue contamination?

25 February 2019 6,556 3 View

Is it possible to multiply more that 2 fuzzy numbers? If yes, what is the principal and also its Matlab function?

There are some methods for multiplication of two fuzzy numbers. But if we want to multiply more that two fuzzy numbers, how we should perform?

21 February 2019 7,893 6 View

What are the most important criteria that sewer network decision-makers should consider during the management planning of these networks?

In addition to the Risk of failure, that consist of the probability and the damage consequences, such as repair cost.

01 June 2017 4,409 5 View

What are the most important principles in defining the green tax related to CO2? What is the suitable amount for water and wastewater services?

For example how much can we receive as a CO2 tax per cubic meter of waste water which is produced?

04 October 2013 3,665 1 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Has anyone used Isolate II RNA mini kit for isolation miRNA from exosomes?

I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...

26 November 2020 9,503 5 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

What is the best RNA isolation method from exosomes of serum samples ?

12 November 2020 9,331 7 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

Could anyone recommend a kit to isolate exosome from cell culture supernatant?

Hello, I'm working with immortalized HUVECs low and I'm trying to study their exosomal RNA contents. Could anyone recommend an effective kit to isolate exosomes from cell culture supernatant?...

31 October 2020 4,252 5 View

How to extract extracellular vesicles from cell lines?

Recently, there was a problem with my qpcr results. I want to try more methods of extracting EV from the cell medium. At present, I am using Invitrogen's kit(Cat4478359) to extract exosomes. Can...

28 October 2020 2,961 3 View

Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

Can we conjugate rabies virus glycoprotein (RVG) directly with exosome?

I have read many articles where researchers get RVG-Exo by transfecting the exosome producing cell through RVG Plasmid. I wanted to ask if anybody knows, can we decorate exosome with RVG directly...

20 October 2020 2,365 2 View