What is the best method to isolate amygdala from the fresh brain in rats?

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Multi band on western blot for glucocorticoid receptor. Which band(s) should I select for quantitative study?

Hello I can see different bands for phosphorylated glucocorticoid receptor protein on the blot membrane. I get confused which band(s) should I select? Can I select the whole areas where the bands...

17 August 2020 6,771 8 View

Is it possible to have machine learning techniques in realtime systems?

Machine Learning (ML) is used to exploit some kind of predictability in the system, while in realtime systems you have to know the exact values and their isn't any place for probabilities. So, do...

07 March 2019 2,027 3 View

Is it possible to multiply more that 2 fuzzy numbers? If yes, what is the principal and also its Matlab function?

There are some methods for multiplication of two fuzzy numbers. But if we want to multiply more that two fuzzy numbers, how we should perform?

21 February 2019 7,893 6 View

What are the most important criteria that sewer network decision-makers should consider during the management planning of these networks?

In addition to the Risk of failure, that consist of the probability and the damage consequences, such as repair cost.

01 June 2017 4,409 5 View

What are the most important principles in defining the green tax related to CO2? What is the suitable amount for water and wastewater services?

For example how much can we receive as a CO2 tax per cubic meter of waste water which is produced?

04 October 2013 3,665 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View