26 February 2019 3 7K Report

I would like to isolate hipocampus and amygdala in rat. what is the best way that I can do it with the least tissue contamination?

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Hi everyone, I am looking for insights into the main cost components of data collection?

Specifically, I am interested in understanding which parts of data collection are most costly. Is it the data collection frequency, the level of expertise required, the data collection methods,...

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How to solve the agglomeration problem with exosomes?

Hi everyone. I am having trouble with my exosomes since they have a high tendency to aggregate, as observed in DLS data and FESEM images (size ranges from 200 nm to 2 micrometer). I have tried...

03 March 2024 9,758 1 View

How do we precipitate a certain plasmid using Poly Ethylene Glycol (PEG)-MgCl2 Method (A problem in optimization) ?

Hello We have a problem in precipitating plasmid DNA (Exactly PIRES2-EGFP) with the PEG-MgCl2 method. The mentioned plasmid after culturing in E.Coli bacteria, harvested and extracted via using of...

11 March 2021 9,659 0 View

Do you kow any biomarker testing laboratory to detect biomarkers related to kappa opioid system?

Hello I am interested to study opioid biomarkers in human blood, especially in kappa opioid system. My main targets are dynorphin and oprk. since the amount of our samples are limited I would...

14 February 2021 3,864 0 View

Multi band on western blot for glucocorticoid receptor. Which band(s) should I select for quantitative study?

Hello I can see different bands for phosphorylated glucocorticoid receptor protein on the blot membrane. I get confused which band(s) should I select? Can I select the whole areas where the bands...

17 August 2020 6,975 8 View

Is it possible to have machine learning techniques in realtime systems?

Machine Learning (ML) is used to exploit some kind of predictability in the system, while in realtime systems you have to know the exact values and their isn't any place for probabilities. So, do...

07 March 2019 2,171 3 View

Is it possible to multiply more that 2 fuzzy numbers? If yes, what is the principal and also its Matlab function?

There are some methods for multiplication of two fuzzy numbers. But if we want to multiply more that two fuzzy numbers, how we should perform?

21 February 2019 8,034 6 View

Is there any rules or suggestions for defining the range of common areas (please check the attached photo) in the fuzzy diagram?

Evidently, the bigger the common area, the greater the uncertainty and vice versa.

10 January 2018 5,061 5 View

What are the most important criteria that sewer network decision-makers should consider during the management planning of these networks?

In addition to the Risk of failure, that consist of the probability and the damage consequences, such as repair cost.

01 June 2017 4,586 5 View

What are the most important principles in defining the green tax related to CO2? What is the suitable amount for water and wastewater services?

For example how much can we receive as a CO2 tax per cubic meter of waste water which is produced?

04 October 2013 3,802 1 View

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