How to set up TETRA-ARMS PCR at higher temperatures?

More Mahboobeh Hemmati's questions See All

Anybody can determine this micro-fossil?

Dear Researchers, I found it from Permian limestone strata in Julfa province (North-West of the Iran country). It should be mentioned, this microfossil extracted from samples collected of the...

29 March 2020 574 5 View

Can somebody help me and recommend publications for extracting Radiolarian microfossils from the Middle to Upper Permian strata?

Dear researchers, I'll be grateful if it is possible to recommend any method papers for extracting Radiolarian microfossils from the Middle to Upper Permian. Sincerely yours, Soheil.

22 March 2020 5,895 4 View

Can anyone suggest what is this microscopic fossil extracted from Middle to Upper Permian limestone strata?

Dear Researchers, I found it from Middle to Upper Permian limestone strata in Julfa NW Iran country. This microfossil extracted form sequences that belong to the Stratigraphy and bio-event...

16 February 2020 3,088 10 View

Anybody knows what is this microscopic fossil from the Middle to Upper Permian limestone strata?

Dear Researchers, Anybody knows what is this microscopic fossil from the Middle to Upper Permian limestone strata? I found it from the Middle to Upper Permian limestone strata in Julfa NW Iran...

26 January 2020 3,108 4 View

How do I choose the major compound of Essential oil?

hi everybody, how do I choose the major compound of Essential oil ? is there any special index or scale for determining the major compound ? what method shall I use ? thanks.

06 December 2019 4,133 2 View

How we can delete some molecules from box in vmd?

I want to delete some solvent molecules in box after md production, but I don't the lables of them and how delete them and how I delete a specific molecule??

11 November 2019 6,415 1 View

Is the water passing through the nafion?

i want to use nafion for desalination.i have a setup that contains two continuous streams of salty and salt free(Passing through Nefion) one side of nafion(cation exchange) is in contact with...

17 June 2019 3,675 5 View

Can any one suggest best and easy method to measure Enzyme activity of glutaminase in breast cancer cell lines?

I search a lot to find the protocol assay for measurment of enzyme activity of glutaminase but i can not find the best method.please help me.

11 April 2019 2,976 2 View

Is it possible if a crystals display inversion symmetry, its second order susceptibility be nonzero?

if a crystals display inversion symmetry, how can its second order susceptibility be non-zero? Thanks alot

30 June 2017 9,889 5 View

How can wash graphene oxide and sonicate it that dont give Precipitate?

how can wash graphene oxide and sonicate it that dont give Precipitate?

30 April 2017 3,942 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Norhidayah Rosman

Hi Mahboobeh Hemmati I suggest you to do the gradient of the annealing temperature to find the best temperature that suits well for the wild-type and the mutant-type primers to get your desired band.

Mahboobeh Hemmati

Hi Norhidayah Rosman

Thank you for your answer

I did. the highest temperature at which all three bands were seen was 65° C.