How to set up TETRA-ARMS PCR at higher temperatures?

Hello everyone .i'm trying to do TETRA ARMS PCR. My primers have TM :64 and one of the primers has TM :66(Reverse outer). How can I setup at higher temperatures to have mutant band? Because at 65...

03 August 2020 4,742 2 View

How do I choose the major compound of Essential oil?

hi everybody, how do I choose the major compound of Essential oil ? is there any special index or scale for determining the major compound ? what method shall I use ? thanks.

06 December 2019 4,133 2 View

How we can delete some molecules from box in vmd?

I want to delete some solvent molecules in box after md production, but I don't the lables of them and how delete them and how I delete a specific molecule??

11 November 2019 6,415 1 View

Can any one suggest best and easy method to measure Enzyme activity of glutaminase in breast cancer cell lines?

I search a lot to find the protocol assay for measurment of enzyme activity of glutaminase but i can not find the best method.please help me.

11 April 2019 2,976 2 View

How to find dynamic coefficient of bearing or seal with Ansys CFX?

Hi everybody In order to rotordynamic analysis at first we solve Reynolds equation (also perturbed Reynolds equation) to find the pressure distribution and consequently we determine the stiffness...

24 December 2018 5,549 3 View

Is it possible if a crystals display inversion symmetry, its second order susceptibility be nonzero?

if a crystals display inversion symmetry, how can its second order susceptibility be non-zero? Thanks alot

30 June 2017 9,889 5 View

How can wash graphene oxide and sonicate it that dont give Precipitate?

how can wash graphene oxide and sonicate it that dont give Precipitate?

30 April 2017 3,942 3 View

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15 May 2016 2,329 10 View

What is the best optimization algorithm such as PSO, SA, GA, FUZZY,... for determination of the optimal generation capacity for renewable energy ?

If you know some article about comparing this algorithms, I would be appreciate to tell me.

17 April 2016 6,686 8 View

How Can I define 3*6 matrix in COMSOL?

15 August 2015 6,522 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Can I use a graph from a another source in a thesis?

I have a permission from X company to use their graph from a report, but they have a reference in the bottom left corner of the graph as follows: "Source: Database Y". Is it OK to use this graph...

24 February 2021 2,269 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Could you please suggest to me research in which a relation between the shear wave velocity and the degree of saturation for stone was discussed?

Hi all, I am looking for a graph or a relation that relates the degree of saturation and the shear wave velocity for the marlstone. Looking forward to your suggestions Best Regards

24 February 2021 9,690 1 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

Algorithms to filter corrective max and min from a set of data?

I've got this 5 year Direct Normal Irradiance data I downloaded from https://re.jrc.ec.europa.eu/. I ordered this by year and by every 24 hours because I need the DNI curve by max in summer, min...

17 February 2021 2,777 5 View

There is any numerical criterion for identifying neighbours for Voronoi /Gabriel graphs?

If there is a set of n points in a plan (with known x, y coordinates), can I use a numerical criterion to establish if two points are neighbours, given the definition of a neighbour for Voronoi...

17 February 2021 6,914 4 View