Hello, I collect blood in gel & clot activator tube for serum separation and I want to extract DNA from clot but unable to separate blood clot from gel as the blood clot lies at the bottom of the tube and is covered with the gel. Please suggest me the method for the separation.
Thanks in advance for all the assistance in this regard!!
Hope dis link vil b useful for U
Article A Simple Method for DNA Isolation from Clotted Blood Extrica...
Dear Apurva
Our preference is that you use a plastic SST Vacutainer® type (serum separation tube) for most tests requiring a serum specimen. Serum separator collection tubes contain a gel that separates the clot from the serum in whole blood specimens. During centrifugation, the gel moves to create a barrier between cells and serum, providing access to the serum for various chemistry, serology and other procedures. Serum separator tubes are recommended for most testing, but please check individual specimen requirements for restrictions.
When using a serum separator tube, collect the specimen using standard venipuncture technique. Gently invert the tube five (5) times to mix the clot activator with the blood. Avoid vigorous agitation of collected specimens. Allow the tube to clot for 20-30 minutes in a vertical position. Do not centrifuge immediately after drawing the blood. Do not allow the blood to clot with the tube in a horizontal position. Do not allow the tube to stand more than one hour before centrifuging.
Centrifuge the tube for 10-15 minutes at about 1300-1800 xg. The centrifuge RPMs will differ depending on the centrifuge and rotor used. Follow the manufacturer's recommendations. The gel barrier will form, separating the serum from the clot. After centrifugation, the gel should be intact and the cells and serum completely separated. Do not re-centrifuge the tube if the barrier is incomplete. Contact the laboratory if you are having difficulty centrifuging specimens.
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts