You might be able to remove the low CMC detergent by mixing it with another protein-friendly detergent that can be removed, such as CHAPS or nOG or DDM.

Hi Adam, I havent understood you. So you suggesting mixed micelles are easy to remove to form liposomes? As of I know LMNG is very hard to exchange with any the other detergent. Even if you extensively wash away during purification you can't remove it completely. Very very slow off rates. Here I have no other option but to use only LMNG because protein gets aggregated in any other milder detergents including the one you suggested.

Shahid Rehan Did you manage to find a solution to this problem?

Hi Dimitrios Kolokouris I have not yet found a robust solution for it. However, I saw a paper where digitonin (with almost similar low cmc as LMNG) was removed using cyclodextran. It may be worth trying. Good luck! https://cyclolab.hu/userfiles/cdn_2017_apr.pdf

Hi Shahid Rehan

an excellent idea. However I feel this is most likely because cyclodextran can capture cholesterols selectively from membranes and digitonin has a cholesterol skeleton. Still it would be worth trying, thanks for the suggestion!

Did some one find the solution how to remove lmng detergent?

Hi, Shahid Rehan and Dimitrios Kolokouris

I've recently tried both beta and gamma cyclodextrin and unfortunately neither of them remove LMNG. It has very similar structure to a lipid molecule, with two carbon tails. I guess this makes sense, since one of cyclodextrin's advantage is that it does not form complex with lipid.