Shahid Rehan

4 Questions 12 Answers 0 Followers

Questions related from Shahid Rehan

Why lipid-mediated dimer of a membrane protein runs lower than the monomer on Native and BN PAGE?

I work on a membrane protein that dimerizes in the presence of certain lipids. We know this from cryoEM structure. I run clear native and BN-PAGE with and without lipids. Some lipids including...

13 December 2023 9,248 1 View

How to remove LMNG detergent to making proteoliposome?

Hi all, I have purified an integral membrane transporter in lauryl maltose neopentyle glycol (LMNG) detergent. Protein seems happy in the detergent and can bind to the inhibitor. However, I want...

23 January 2018 4,661 7 View

Plasma membrane isolation from insect cells using sucrose gradiant?

Hi, I'm trying to purify couple of integral membrane proteins from Sf21 cells. Typically I isolate total membrane (PM, ER, golgi etc) after cell lysis and couple of centrifugations. Additionally I...

06 December 2016 8,595 2 View

Any advice on double band in ingel flourescence fig 5.?

Is a double band in ingel flourescence fig 5 in most of the lanes a gel artifact or is it a glycosylated form of the protein?

16 December 2013 8,971 1 View