Shahid Rehan

6 Questions 16 Answers 0 Followers

Questions related from Shahid Rehan

Why lipid-mediated dimer of a membrane protein runs lower than the monomer on Native and BN PAGE?

I work on a membrane protein that dimerizes in the presence of certain lipids. We know this from cryoEM structure. I run clear native and BN-PAGE with and without lipids. Some lipids including...

13 December 2023 9,321 1 View

How to remove LMNG detergent to making proteoliposome?

Hi all, I have purified an integral membrane transporter in lauryl maltose neopentyle glycol (LMNG) detergent. Protein seems happy in the detergent and can bind to the inhibitor. However, I want...

23 January 2018 4,713 7 View

Plasma membrane isolation from insect cells using sucrose gradiant?

Hi, I'm trying to purify couple of integral membrane proteins from Sf21 cells. Typically I isolate total membrane (PM, ER, golgi etc) after cell lysis and couple of centrifugations. Additionally I...

06 December 2016 8,663 2 View

What are detergent off rates and how it can affect membrane protein dynamics?

Hi all, I am little confused about detergent's off rates. What does that mean when we say one detergent has lower or higher off rates than the other?. And is there any correlation between...

11 August 2016 211 3 View

How sucrose of glucose reduces the protease release?

Sucrose or glucose are often constituent of cell lysis buffer. It is known to protect protein/enzymes from proteases. But I would like to know how exactly this happens?

20 April 2016 4,198 4 View

Any advice on double band in ingel flourescence fig 5.?

Is a double band in ingel flourescence fig 5 in most of the lanes a gel artifact or is it a glycosylated form of the protein?

16 December 2013 9,026 1 View