1. How to remove bubbles during preparation of agarose gel or after prepared it?
2. When we inject the gel inside the capillary tube, there are some bubbles visible. What is the way to remove those bubbles?
I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.
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give some examples or add some case studies for a better explanation
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