I have 5 M Nacl , 2M Buffer and 30% Glycerol
How to prepare 500 ml of solution containing 400 mM Nacl and 50 mM of buffer.
Thanks in advance.
If I understand your question correctly use CV to calculate the NaCl and Buffer content you need.
Then find out the total volume of both and reduced by 500 mal which is the volume of Glycerol.
I believe you wanna prepare the solution in 30% Glycerol
Just do as M. Farooq Wahab
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