How to reduce the molarity and prepare a solution?

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Udaya Jayasundara

If I understand your question correctly use CV to calculate the NaCl and Buffer content you need.

Then find out the total volume of both and reduced by 500 mal which is the volume of Glycerol.

I believe you wanna prepare the solution in 30% Glycerol

Mário Silva

Just do as M. Farooq Wahab

suggests: Cinitial x Vinitial = Cfinal x Vfinal. The universal mass balance as you learned in high-school.