What are the parameters affecting the binding time of aptamer besides temperature? The binding buffer concentration? Can anyone help? Thanks very much.
Enhancing the temperature which accelerates the diffusion, thus reduces the binding time.
Dear Ma,
You can change and investigate the environmental conditions such as temperature, pH, and buffer concentration in order to optimize the
binding time of aptamer and target.
Best regards,
Thanks all. Zhaoxi Sun, Jon Ashley, Asghar Narmani. I will try the parameters you guys mentioned.
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