Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

In the paper mentioned can you help me to find the finite difference of equation no 19.?

In the paper "Magnetometer-Only Attitude Determination Using Novel Two-Step Kalman Filter Approach" in equation 19 do I need to complete quaternion multiplication first and then do simple...

15 June 2020 7,522 4 View

How to align reverse and forward DNA sequences in chromus software ??

Need detailed procedure to align DNA seq. before performing BLAST.

09 January 2019 7,584 3 View

For detecting single nucleotide mutation, do we use traditional PCR or qPCR?

I have got a deletion of single base pair thymine at 3744 position in BRCA1 gene, which PCR technique should I use to detect our gene mutation ?

05 March 2018 3,492 3 View

How to calculate grafting % for two polymers?

Hello, I am performing grafting of two polymers using microwave assisted method. I want to know that what is the correct method to assess and calculate grafting % among the two polymers. Is...

04 February 2018 6,874 3 View

How can you ensure that the drug is completely separated from liposomes?

After doing either sephadex column or ultrafiltration. How do you ensure that entire unentrapped drug is removed from liposomes? Will there be any extra peak in DLS?

10 January 2018 1,293 6 View

Can you suggest best cost effective CO2 incubator selling company for animal cell culture?

Hi I am looking for best cost effective CO2 incubator for animal cell culture facility. There are lot of models by various companies with different features. As such, so many options and hence, a...

20 August 2017 1,957 7 View

I want to study rearrangement of ROS 1 and RET genes in FFPE sample?

24 December 2016 4,778 1 View

What is the actual molecular weight of CD63 for exosome identification?

Hii, I always get variable result for CD63 immunoblot from exosomal protein as well as cellular protein of RAW cell line macrophages.I found some paper where it mentioned that CD63 has alternative...

29 November 2016 4,536 0 View

Which solvent out of Hexane and Acetone is more preferable for dissolution of FAME?

what is the solubility of FAME in acetone ?

02 December 2015 8,607 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View