After doing either sephadex column or ultrafiltration. How do you ensure that entire unentrapped drug is removed from liposomes? Will there be any extra peak in DLS?
Depends on the drug and the spectro-chromatography technique you are using to which drug responds and is detected or 'seen'.
it can be used gel filtration chromatography. I haven't had much success with ultracentrifugation. Either the liposomes float, or they form a rather loose pellet. Either way, it's hard to get a good separation from the liquid, and the yield can be poor. Gel filtration columns are easy to make and fast to run. When working on a small scale, I make 0.8 x 20-cm columns, and I don't reuse the resin, saving the trouble of cleaning it. The only disadvantage is that the sample gets diluted. This problem is avoided by dialysis, but that takes much longer.
I don't think there will be any extra peak on DLS, but you may find drug characteristic peaks on UV. You may also find fast initial release if you run release experiment .
Hi Bin. The diameter of liposomes I made is around 200 nm. I only used ultrafiltration so far. And it depends on the Molecular weight cut off of the filter you choose to remove the drug. Sorry I never used sephadex column so far.
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