During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the protein to be in cytoplasm and in WoLF PSORT, it identified the same protein to be in the endoplasmic reticulum. Why is this difference?
Hi Versha, this online software runs on different Algorithms, as different algorithms have different ways to predict and sort the data, sometimes the prediction changes.
CELLO uses a two-level Support Vector Machine system to assign localizations.
WoLF PSORT is an extension of the PSORT II program for protein subcellular location prediction.
Some like PSORT db - uses laboratory experimentation (ePSORTdb dataset) and computational predictions (cPSORTdb dataset) data.
Cheers,
Dharmesh
Maybe there are different isoforms of the protein. (Is it the only difference?) Are the proteins the same? Do they do the same? Maybe is just the database.
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